P-11 Response of pancreatic ductal adenocarcinoma to gemcitabine using a human organotypic slice culture model
نویسندگان
چکیده
The organotypic tumor slice culture model resembles the in vivo situation of tumors. It may serve as a good ex platform for response to anti-tumor therapies. We aimed evaluate pancreatic ductal adenocarcinoma (PDA) cells and helper, cytotoxic, regulatory T gemcitabine using human PDA model. tissues were obtained from patients who operated on resection. A vibratome sliced tumors, slices cultured until 5 days with 0.1 μM, 1 10 μM or 2 days. Tumor responses evaluated by number proportion Ki-67 caspase-3 positively expressed tumor. CD4+, CD8+, FOXP3+ analyzed immunohistochemistry staining. Gemcitabine treatment exerted proportional cytotoxic effects terms cell number, cleaved expression. Mean positive decreased according increase concentration (48h treatment; 65.7% control vs. 27.9% p=0.025). increased 8.6% 18.6% p=0.042). per x200 78.3 1.0 27.3 cells/total concentration; 24h treatment, mean CD4+ 2.6%, 1.6%, 1.5%, 0.5%, CD8+ 1.4%, 1.1%, 0.2%, 0.3%, 0.1% control, gemcitabine, respectively. However, CD8+/FOXP3+ ratio remained stable 4.4 8.6 (p=0.039). tumoricidal effect could be demonstrated however, increased.
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ژورنال
عنوان ژورنال: Annals of Oncology
سال: 2023
ISSN: ['0923-7534', '1569-8041']
DOI: https://doi.org/10.1016/j.annonc.2023.04.067